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Figure 2
Pretreatment with curcumin blunts cardiac hypertrophy in vivo. (A and C) Statistical results of HW/BW ratio, LW/BW ratio, and myocyte cross-sectional areas (n = 200 cells per section) at 8 weeks after AB surgery (A; n = 6) or 3 weeks after PE infusion (C; n = 7). (B and D) Gross heart and WGA-FITC staining of sham and AB mice at 8 weeks after surgery (B) or 3 weeks of saline- and PE-infused mice (D) treated with or without curcumin. Scale bars: 20 mm (gross heart); 50 μm (WGA stain). (E and F) Analysis of hypertrophic markers (n = 4). Total RNA was isolated from hearts of mice of the indicated groups, and expression of transcripts for ANP, BNP, β-MHC, and α-MHC induced by AB (E) or PE infusion (F) was determined by Northern blot analysis. *P < 0.05 versus respective vehicle control.