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Figure 6
Pretreatment with curcumin inhibits inflammation and fibrosis induced by AB. (A) Quantitative analysis revealed the population of MPO-, Mac-1–, and Mac-3–positive cells in the hearts of the indicated mice (n = 6). (B) Western blot analysis of TNF-α, IL-1β, IL-6, and MCP-1 protein expression in the myocardia obtained from the indicated mice (n = 6). Each assay was performed in triplicate. (C) PSR staining on histological sections of the LV was performed on each group 8 weeks after AB. Scale bars: 10 μm. (D) Fibrotic areas from histological sections were quantified using an image-analyzing system (n = 6). (E and F) Northern blot and Western blot analyses of TGF-β1, collagen I, and CTGF were performed to determine mRNA (E) and protein (F) expression levels in each group 8 weeks after AB (n = 3). GAPDH was used as the sample loading control. *P < 0.05 versus vehicle-treated sham control.