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Figure 2
Intranasal leptin inhibits in vivo PTZ-induced generalized convulsive seizures and elevates serum and brain leptin levels in mice. (A) In 6- to 8-week-old CD-1 mice, 800 μg/kg leptin (n = 15) given i.n. 30 minutes before the i.p. injection of 75 mg/kg PTZ significantly increased the latency to behaviorally assessed generalized clonic-tonic seizures. *P < 0.002 compared with mice that received vehicle ( n = 16). (B) Cortex leptin per milligram of cortex protein (*P < 0.001). (C) Serum leptin concentrations increased significantly (*P < 0.0001) after 800 μg/kg leptin i.n. compared with vehicle. The Mann-Whitney U test was used for the statistical analysis.