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Figure 5
rAAV-induced gag-specific CD8⁺ T cells provide partial protection against challenge with Vacgag. (A) To test for protection, groups of nonimmunized mice and mice immunized with 10¹¹ gc rAAV2/7gag or 10⁹ vp AdC68gag were challenged 1 month after vaccination with Vacgag. After 5 days, ovaries were harvested and vaccinia virus titers were determined. (B) PBMCs prior to challenge and PBMCs and splenocytes following challenge were analyzed by ICS for frequencies of gag-specific IFN-γ–secreting CD8⁺ T cells. Background frequencies (less than 0.1%) were subtracted prior to plotting. Data are representative of 3 repeated experiments.