Neutrophil gelatinase–associated lipocalin mediates 13-cis retinoic acid–induced apoptosis of human sebaceous gland cells
J. Clin. Invest. Amanda M. Nelson, et al. 118:1468 doi:10.1172/JCI33869 [
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Figure 7siRNA to NGAL blocks the induction of cleaved caspase 3 by 13-
cis RA in SEB-1 sebocytes.
Under siRNA conditions described in Figure
6, immunoblotting revealed that siRNA to
LCN2 decreases expression of cleaved caspase 3 by approximately 5-fold compared with siRNA control. Blots were incubated with primary antibodies to cleaved caspase 3 as well as β-actin as a loading control to normalize densitometry values. Three independent scrambled siCONTROL (SCRAM) and LCN2 western blots are shown. p17 and p19 are the cleaved or active fragments of caspase 3. Graph represents the normalized expression of cleaved caspase 3 from 5 independent western blots. These data indicate that NGAL mediates the apoptotic response of SEB-1 sebocytes to 13-
cis RA. Statistical analysis was performed with paired
t test, mean ± SEM; *
P < 0.05.
