Mouse ES cell–derived cardiac precursor cells are multipotent and facilitate identification of novel cardiac genes
J. Clin. Invest. Nicolas Christoforou, et al. 118:894 doi:10.1172/JCI33942 [
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Figure 1Examination of CPC presence in cultures of differentiating mES cells temporally (qRT-PCR). Relative RNA levels of genes associated with cardiac differentiation in EBs as measured by qRT-PCR.
Brachyury expression is associated with mesoderm formation. The transcription factors
Nkx2-5 and
Tbx5 are indicators of initiation of cardiac development, and the structural proteins
Myh6 and
Tnnt2 are expressed in functional cardiomyocytes. Error bars indicate SD.