Abstract Chemokines (eg, IL-8) were originally identified as chemotactic proteins obtained
from various different natural sources. Today, using the genome walking strategy an ever-
increasing number of novel genes encoding chemokines have been discovered that were
expressed in bacteria or eukaryotic cells and subsequently tested for biological activity.
Usually biological significance of the considered chemokine is extrapolated from these data.
The increasing evidence, however, that post-translational modification of chemokines can …

Abstract Chemokines (eg, IL-8) were originally identified as chemotactic proteins obtained
from different natural sources. These were purified by the use of a bioassay followed by N-
terminal sequencing and then molecular cloning giving the full sequence and making it
possible to express the chemokine as a recombinant protein. In this way we were sure that
the biologically active chemokine is really released from natural sources. Today, using the
genome walking strategy, we have discovered an ever-increasing number of novel genes …