[HTML][HTML] Identification of WNK1 as a substrate of Akt/protein kinase B and a negative regulator of insulin-stimulated mitogenesis in 3T3-L1 cells
Insulin signaling through protein kinase Akt/protein kinase B (PKB), a downstream element
of the phosphatidylinositol 3-kinase (PI3K) pathway, regulates diverse cellular functions
including metabolic pathways, apoptosis, mitogenesis, and membrane trafficking. To identify
Akt/PKB substrates that mediate these effects, we used antibodies that recognize
phosphopeptide sites containing the Akt/PKB substrate motif (RXRXX (p) S/T) to
immunoprecipitate proteins from insulin-stimulated adipocytes. Tryptic peptides from a 250 …
of the phosphatidylinositol 3-kinase (PI3K) pathway, regulates diverse cellular functions
including metabolic pathways, apoptosis, mitogenesis, and membrane trafficking. To identify
Akt/PKB substrates that mediate these effects, we used antibodies that recognize
phosphopeptide sites containing the Akt/PKB substrate motif (RXRXX (p) S/T) to
immunoprecipitate proteins from insulin-stimulated adipocytes. Tryptic peptides from a 250 …