[HTML][HTML] Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress
IM Ward, J Chen - Journal of Biological Chemistry, 2001 - ASBMB
H2AX, a member of the histone H2A family, is rapidly phosphorylated in response to ionizing
radiation. This phosphorylation, at an evolutionary conserved C-terminal
phosphatidylinositol 3-OH-kinase-related kinase (PI3KK) motif, is thought to be critical for
recognition and repair of DNA double strand breaks. Here we report that inhibition of DNA
replication by hydroxyurea or ultraviolet irradiation also induces phosphorylation and foci
formation of H2AX. These phospho-H2AX foci colocalize with proliferating cell nuclear …
radiation. This phosphorylation, at an evolutionary conserved C-terminal
phosphatidylinositol 3-OH-kinase-related kinase (PI3KK) motif, is thought to be critical for
recognition and repair of DNA double strand breaks. Here we report that inhibition of DNA
replication by hydroxyurea or ultraviolet irradiation also induces phosphorylation and foci
formation of H2AX. These phospho-H2AX foci colocalize with proliferating cell nuclear …