[HTML][HTML] The Bloom's syndrome helicase unwinds G4 DNA
H Sun, JK Karow, ID Hickson, N Maizels - Journal of Biological Chemistry, 1998 - ASBMB
BLM, the gene that is defective in Bloom's syndrome, encodes a protein homologous to
RecQ subfamily helicases that functions as a 3′-5′ DNA helicase in vitro. We now report
that the BLM helicase can unwind G4 DNA. The BLM G4 DNA unwinding activity is ATP-
dependent and requires a short 3′ region of single-stranded DNA. Strikingly, G4 DNA is a
preferred substrate of the BLM helicase, as measured both by efficiency of unwinding and by
competition. These results suggest that G4 DNA may be a natural substrate of BLM in vivo …
RecQ subfamily helicases that functions as a 3′-5′ DNA helicase in vitro. We now report
that the BLM helicase can unwind G4 DNA. The BLM G4 DNA unwinding activity is ATP-
dependent and requires a short 3′ region of single-stranded DNA. Strikingly, G4 DNA is a
preferred substrate of the BLM helicase, as measured both by efficiency of unwinding and by
competition. These results suggest that G4 DNA may be a natural substrate of BLM in vivo …