[HTML][HTML] Human werner syndrome DNA helicase unwinds tetrahelical structures of the fragile X syndrome repeat sequence d (CGG) n
Formation of hairpin and tetrahelical structures by ad (CGG) trinucleotide repeat sequence is
thought to cause expansion of this sequence and to engender fragile X syndrome. Here we
show that human Werner syndrome DNA helicase (WRN), a member of the RecQ family of
helicases, efficiently unwinds G′ 2 bimolecular tetraplex structures of d (CGG) 7.
Unwinding of d (CGG) 7 by WRN requires hydrolyzable ATP and Mg 2+ and is proportional
to the amount of added helicase and to the time of incubation. The efficiencies of unwinding …
thought to cause expansion of this sequence and to engender fragile X syndrome. Here we
show that human Werner syndrome DNA helicase (WRN), a member of the RecQ family of
helicases, efficiently unwinds G′ 2 bimolecular tetraplex structures of d (CGG) 7.
Unwinding of d (CGG) 7 by WRN requires hydrolyzable ATP and Mg 2+ and is proportional
to the amount of added helicase and to the time of incubation. The efficiencies of unwinding …