[HTML][HTML] Heparan sulfate mediates bFGF transport through basement membrane by diffusion with rapid reversible binding
CJ Dowd, CL Cooney, MA Nugent - Journal of Biological Chemistry, 1999 - ASBMB
Basic fibroblast growth factor (bFGF) is a pluripotent cytokine with a wide range of target
cells. Heparan sulfate binds bFGF, and this interaction has been demonstrated to protect
bFGF against physical denaturation and protease degradation. The high concentrations of
heparan sulfate in basement membranes have implicated these matrices as storage sites for
bFGF in vivo. However, the mechanisms by which basement membranes modulate bFGF
storage and release is unknown. To gain insight into these mechanisms, we have …
cells. Heparan sulfate binds bFGF, and this interaction has been demonstrated to protect
bFGF against physical denaturation and protease degradation. The high concentrations of
heparan sulfate in basement membranes have implicated these matrices as storage sites for
bFGF in vivo. However, the mechanisms by which basement membranes modulate bFGF
storage and release is unknown. To gain insight into these mechanisms, we have …