Role of the integrin alpha v beta 6 in cell attachment to fibronectin. Heterologous expression of intact and secreted forms of the receptor.
A Weinacker, A Chen, M Agrez, RI Cone… - Journal of Biological …, 1994 - ASBMB
The integrin alpha v beta 6 has been shown to be a fibronectin-binding protein. To
determine whether the cytoplasmic and transmembrane domains of alpha v beta 6 are
necessary for binding to fibronectin, a truncated, secreted form of the integrin lacking these
domains was engineered and expressed in Chinese hamster ovary cells. Fibronectin affinity
chromatography demonstrated that the secreted integrin, like its full-length counterpart, was
capable of binding fibronectin. Monoclonal antibodies were made to secreted alpha v beta 6 …
determine whether the cytoplasmic and transmembrane domains of alpha v beta 6 are
necessary for binding to fibronectin, a truncated, secreted form of the integrin lacking these
domains was engineered and expressed in Chinese hamster ovary cells. Fibronectin affinity
chromatography demonstrated that the secreted integrin, like its full-length counterpart, was
capable of binding fibronectin. Monoclonal antibodies were made to secreted alpha v beta 6 …