[HTML][HTML] Regulation of 5′-AMP-activated protein kinase activity by the noncatalytic β and γ subunits
JRB Dyck, G Gao, J Widmer, D Stapleton… - Journal of Biological …, 1996 - ASBMB
The mammalian 5′-AMP-activated protein kinase is a heterotrimer consisting of an α
catalytic subunit and β and γ noncatalytic subunits, each of which is represented in a larger
isoprotein family, related to the SNF1 kinase and its interacting proteins in yeast. In this
study, we have used mammalian cell transfection to compare the activities of the two α
subunit isoforms, α-1 and α-2, and to study the influence of the noncatalytic subunits on
enzyme subunit association and activity. Expression of epitope-tagged protein subunits in …
catalytic subunit and β and γ noncatalytic subunits, each of which is represented in a larger
isoprotein family, related to the SNF1 kinase and its interacting proteins in yeast. In this
study, we have used mammalian cell transfection to compare the activities of the two α
subunit isoforms, α-1 and α-2, and to study the influence of the noncatalytic subunits on
enzyme subunit association and activity. Expression of epitope-tagged protein subunits in …