[HTML][HTML] The carboxyl terminus of the cystic fibrosis transmembrane conductance regulator binds to AP-2 clathrin adaptors
KM Weixel, NA Bradbury - Journal of Biological Chemistry, 2000 - ASBMB
The cystic fibrosis transmembrane conductance regulator (CFTR) undergoes rapid and
efficient endocytosis. Since functionally active CFTR is found in purified clathrin-coated
vesicles isolated from both cultured epithelial cells and intact epithelial tissues, we
investigated the molecular mechanisms whereby CFTR could enter such endocytic clathrin-
coated vesicles. In vivocross-linking and in vitro pull-down assays show that full-length
CFTR binds to the endocytic adaptor complex AP-2. Fusion proteins containing the carboxyl …
efficient endocytosis. Since functionally active CFTR is found in purified clathrin-coated
vesicles isolated from both cultured epithelial cells and intact epithelial tissues, we
investigated the molecular mechanisms whereby CFTR could enter such endocytic clathrin-
coated vesicles. In vivocross-linking and in vitro pull-down assays show that full-length
CFTR binds to the endocytic adaptor complex AP-2. Fusion proteins containing the carboxyl …