[HTML][HTML] Na+/H+ exchanger NHE3 activity and trafficking are lipid Raft-dependent
A previous study showed that∼ 25–50% of rabbit ileal brush border (BB) Na+/H+ exchanger
NHE3 is in lipid rafts (LR)(Li, X., Galli, T., Leu, S., Wade, JB, Weinman EJ, Leung, G.,
Cheong, A., Louvard, D., and Donowitz, M.(2001) J. Physiol.(Lond.) 537, 537–552). Here,
we examined the role of LR in NHE3 transport activity using a simpler system: opossum
kidney (OK) cells (a renal proximal tubule epithelial cell line) containing NHE3.∼ 50% of
surface (biotinylated) NHE3 in OK cells distributed in LR by density gradient centrifugation …
NHE3 is in lipid rafts (LR)(Li, X., Galli, T., Leu, S., Wade, JB, Weinman EJ, Leung, G.,
Cheong, A., Louvard, D., and Donowitz, M.(2001) J. Physiol.(Lond.) 537, 537–552). Here,
we examined the role of LR in NHE3 transport activity using a simpler system: opossum
kidney (OK) cells (a renal proximal tubule epithelial cell line) containing NHE3.∼ 50% of
surface (biotinylated) NHE3 in OK cells distributed in LR by density gradient centrifugation …