Detection and plasma pharmacokinetics of an anti-vascular endothelial growth factor oligonucleotide-aptamer (NX1838) in rhesus monkeys
CE Tucker, LS Chen, MB Judkins, JA Farmer… - … of Chromatography B …, 1999 - Elsevier
Aptamers are oligonucleotide ligands selected, in vitro, to bind a specified target protein.
The first aptamer to reach human clinical testing is NX1838, a polyethylene glycol
conjugated aptamer that inhibits vascular endothelial growth factor. This paper describes the
validation of a high-performance liquid chromatographic anion-exchange method for the
determination of NX1838 in plasma. Measurements of intact NX1838 had a coefficient of
variation of less than 8% and an accuracy between 107% and 115%. The assay was utilized …
The first aptamer to reach human clinical testing is NX1838, a polyethylene glycol
conjugated aptamer that inhibits vascular endothelial growth factor. This paper describes the
validation of a high-performance liquid chromatographic anion-exchange method for the
determination of NX1838 in plasma. Measurements of intact NX1838 had a coefficient of
variation of less than 8% and an accuracy between 107% and 115%. The assay was utilized …