[HTML][HTML] Palmitate-induced apoptosis can occur through a ceramide-independent pathway
LL Listenberger, DS Ory, JE Schaffer - Journal of Biological Chemistry, 2001 - ASBMB
Cytotoxic accumulation of long chain fatty acids has been proposed to play an important role
in the pathogenesis of diabetes mellitus and heart disease. To explore the mechanism of
cellular lipotoxicity, we cultured Chinese hamster ovary cells in the presence of media
supplemented with fatty acid. The saturated fatty acid palmitate, but not the
monounsaturated fatty acid oleate, induced programmed cell death as determined by
annexin V positivity, caspase 3 activity, and DNA laddering. De novo ceramide synthesis …
in the pathogenesis of diabetes mellitus and heart disease. To explore the mechanism of
cellular lipotoxicity, we cultured Chinese hamster ovary cells in the presence of media
supplemented with fatty acid. The saturated fatty acid palmitate, but not the
monounsaturated fatty acid oleate, induced programmed cell death as determined by
annexin V positivity, caspase 3 activity, and DNA laddering. De novo ceramide synthesis …