[HTML][HTML] Regulation of the p70 S6 kinase by phosphorylation in vivo: analysis using site-specific anti-phosphopeptide antibodies
QP Weng, M Kozlowski, C Belham, A Zhang… - Journal of Biological …, 1998 - ASBMB
The p70 S6 kinase is activated by diverse stimuli through a multisite phosphorylation
directed at three separate domains as follows: a cluster of (Ser/Thr) Pro sites in an
autoinhibitory segment in the noncatalytic carboxyl-terminal tail; Thr-252 in the activation
loop of the catalytic domain; and Ser-394 and Thr-412 in a segment immediately carboxyl-
terminal to the catalytic domain. Phosphorylation of Thr-252 in vitro by the enzyme
phosphatidylinositol 3-phosphate-dependent kinase-1 or mutation of Thr-412→ Glu has …
directed at three separate domains as follows: a cluster of (Ser/Thr) Pro sites in an
autoinhibitory segment in the noncatalytic carboxyl-terminal tail; Thr-252 in the activation
loop of the catalytic domain; and Ser-394 and Thr-412 in a segment immediately carboxyl-
terminal to the catalytic domain. Phosphorylation of Thr-252 in vitro by the enzyme
phosphatidylinositol 3-phosphate-dependent kinase-1 or mutation of Thr-412→ Glu has …