[HTML][HTML] Modulation by peroxynitrite of Akt-and AMP-activated kinase-dependent Ser1179 phosphorylation of endothelial nitric oxide synthase
Peroxynitrite (ONOO−), a nitric oxide-derived oxidant, uncouples endothelial nitric oxide
synthase (eNOS) and increases enzymatic production of superoxide anions (O 2 O)(Zou,
MH, Shi, C., and Cohen, RA (2002) J. Clin. Invest. 109, 817–826). Here we studied how
ONOO− influences eNOS activity. In cultured bovine aortic endothelial cells (BAEC), ONOO−
increased basal and agonist-stimulated Ser 1179 phosphorylation of eNOS, whereas it
decreased nitric oxide production and bioactivity. However, ONOO− strongly inhibited the …
synthase (eNOS) and increases enzymatic production of superoxide anions (O 2 O)(Zou,
MH, Shi, C., and Cohen, RA (2002) J. Clin. Invest. 109, 817–826). Here we studied how
ONOO− influences eNOS activity. In cultured bovine aortic endothelial cells (BAEC), ONOO−
increased basal and agonist-stimulated Ser 1179 phosphorylation of eNOS, whereas it
decreased nitric oxide production and bioactivity. However, ONOO− strongly inhibited the …