MATERIALS AND METHODS

The protocol of this study was approved by the local Ethics Committee of Firat University, Faculty of Medicine. Adult male BALB/c mice (30–35 g) were divided into two groups (n= 6 per each group). The animals were obtained from the Firat University Medical School Experimental Research Unit where they were housed in a temperature-controlled environment (21±1 C) with lights on between 07.00 and 19.00. Food and water were supplied ad libitum. Leptin was dissolved in phosphate buffered saline (PBS)(1 mg/ml) and intraperitoneally administered group at a dose of 40 μg/animal for 5 days. Control group received PBS. The volume of injected leptin solution or PBS was 100 μl. All animals were decapitated one hour after the last injection. Brain tissues were rapidly removed and kept in dry ice. Total brain tissues were homogenized in PBS. Lipid hydroperoxide (which reflects the degree of lipid peroxidation better than other peroxidation products such as malondialdehyde) and GSH levels were measured by enzyme immunoassay using specific kits (Cayman Chemical Co., MI, USA). Data were expressed as mean±SEM and statistically analyzed by using Mann Whitney’s U-test. P< 0.05 was considered statistically significant.