A simple method is described for the isolation of human peripheral blood eosinophils by an
immunomagnetic procedure. Superparamagnetic particles were coupled to a monoclonal
antibody against CD16, a molecule present on neutrophils but not on eosinophils. A
peripheral blood granulocyte preparation, containing neutrophils and eosinophils, was
incubated with these anti-CD16 particles. In the magnetic field of a permanent magnet,
magnetically labelled neutrophils were then retained on columns with a ferromagnetic …