[CITATION][C] Interaction of Mesangial Cells with IgA and IgG Immune Complexes: A Possible Mechanism of Glomerular Injury in IgA Nephropathy1
C Gómez-Guerrero, E González, P Hernando… - IgA Nephropathy: The …, 1993 - karger.com
IgA Nephropathy: The 25th Year, 1993•karger.com
Materials and Methods Isolation, Desialylation and Radiolabeling of Proteins Human IgA
and IgG were isolated from the sera of patients with myeloma as previously described [10].
Both IgA and IgG preparations were found to be free of other immunoglobulins as judged by
immunoelectrophoresis and double immunodiffusion analysis. Purified IgA was desialylated
as described [11]. The extent of desialylation was monitored by the thiobarbituric assay [12].
Approximately 70% of sialic acid was removed from IgA. IgA and desialylated IgA (desIgA) …
and IgG were isolated from the sera of patients with myeloma as previously described [10].
Both IgA and IgG preparations were found to be free of other immunoglobulins as judged by
immunoelectrophoresis and double immunodiffusion analysis. Purified IgA was desialylated
as described [11]. The extent of desialylation was monitored by the thiobarbituric assay [12].
Approximately 70% of sialic acid was removed from IgA. IgA and desialylated IgA (desIgA) …
Materials and Methods
Isolation, Desialylation and Radiolabeling of Proteins Human IgA and IgG were isolated from the sera of patients with myeloma as previously described [10]. Both IgA and IgG preparations were found to be free of other immunoglobulins as judged by immunoelectrophoresis and double immunodiffusion analysis. Purified IgA was desialylated as described [11]. The extent of desialylation was monitored by the thiobarbituric assay [12]. Approximately 70% of sialic acid was removed from IgA. IgA and desialylated IgA (desIgA) were radiolabeled with 1 mCi Na125I by the chloramine T method to a specific activity of 0.1 mCi/mg.
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