Glycosylation of circulating IgA in patients with IgA nephropathy modulates proliferation and apoptosis of mesangial cells
A Amore, P Cirina, G Conti, P Brusa… - Journal of the …, 2001 - journals.lww.com
Abnormalities in circulating IgA1 have been demonstrated in patients with IgA nephropathy
(IgAN). This study addresses the question of the functional significance of this alteration in
creating mesangial injury. Biologic effects of selected IgA glycoforms isolated from serum of
IgAN patients and controls and in vitro deglycosylated normal IgA were tested on cultured
human mesangial cells (MC). IgA glycoforms, ranging from 250 to 500 kD molecular weight,
were isolated by lectin affinity chromatography followed by HPLC. IgA and IgG content was …
(IgAN). This study addresses the question of the functional significance of this alteration in
creating mesangial injury. Biologic effects of selected IgA glycoforms isolated from serum of
IgAN patients and controls and in vitro deglycosylated normal IgA were tested on cultured
human mesangial cells (MC). IgA glycoforms, ranging from 250 to 500 kD molecular weight,
were isolated by lectin affinity chromatography followed by HPLC. IgA and IgG content was …