[HTML][HTML] The potential role of proteoglycans in cationic lipid-mediated gene delivery: studies of the interaction of cationic lipid-DNA complexes with model …
CM Wiethoff, JG Smith, GS Koe… - Journal of Biological …, 2001 - ASBMB
Recent evidence supports a role for proteoglycans in polycation-mediated gene delivery.
Therefore, the interaction of glycosaminoglycans with cationic lipid-DNA complexes
(CLDCs) has been characterized using a combination of biophysical approaches. At low
ionic strength, CLDCs bind to heparin-derivatized Sepharose particles, with the ratio of
cationic lipid to DNA controlling the binding. Incorporation of the helper lipids cholesterol or
1, 2-dioleoyl-phosphatidylethanolamine increases the amount of bound CLDC. Heparin also …
Therefore, the interaction of glycosaminoglycans with cationic lipid-DNA complexes
(CLDCs) has been characterized using a combination of biophysical approaches. At low
ionic strength, CLDCs bind to heparin-derivatized Sepharose particles, with the ratio of
cationic lipid to DNA controlling the binding. Incorporation of the helper lipids cholesterol or
1, 2-dioleoyl-phosphatidylethanolamine increases the amount of bound CLDC. Heparin also …