Production of α-1, 3-galactosyltransferase knockout pigs by nuclear transfer cloning
L Lai, D Kolber-Simonds, KW Park, HT Cheong… - Science, 2002 - science.org
L Lai, D Kolber-Simonds, KW Park, HT Cheong, JL Greenstein, GS Im, M Samuel, A Bonk…
Science, 2002•science.orgThe presence of galactose α-1, 3-galactose residues on the surface of pig cells is a major
obstacle to successful xenotransplantation. Here, we report the production of four live pigs in
which one allele of the α-1, 3-galactosyltransferase locus has been knocked out. These pigs
were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as
nuclear donors for embryos reconstructed with enucleated pig oocytes.
obstacle to successful xenotransplantation. Here, we report the production of four live pigs in
which one allele of the α-1, 3-galactosyltransferase locus has been knocked out. These pigs
were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as
nuclear donors for embryos reconstructed with enucleated pig oocytes.
The presence of galactose α-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the α-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.
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