A three-dimensional model of differentiation of immortalized human bronchial epithelial cells
MB Vaughan, RD Ramirez, WE Wright, JD Minna… - Differentiation, 2006 - Elsevier
A therapeutic approach being investigated for a variety of pathologies is tissue regeneration
using a patient's own cells. Such studies have been hampered due to the difficulty in
growing epithelial cells for prolonged periods in culture. Replicative senescence due to
short telomeres and p16 induced by culture stress work together to inhibit cell growth.
Forced expression of telomerase (hTERT) can prevent replicative senescence, and
expression of the cell cycle protein cdk4 can sequester p16, thereby immortalizing epithelial …
using a patient's own cells. Such studies have been hampered due to the difficulty in
growing epithelial cells for prolonged periods in culture. Replicative senescence due to
short telomeres and p16 induced by culture stress work together to inhibit cell growth.
Forced expression of telomerase (hTERT) can prevent replicative senescence, and
expression of the cell cycle protein cdk4 can sequester p16, thereby immortalizing epithelial …
