The packaging of DNA with histones to form chromatin represents an important and powerful
mechanism to regulate gene expression. Critical aspects of chromatin-specific contributions
to gene regulation have been revealed by the comparison of the activities from DNA
regulatory elements examined both as transiently transfected reporters and stably integrated
reporters organized as chromatin. Using the mouse mammary tumor virus (MMTV) promoter
as a model, we probed the structural differences between transiently transfected and stably …