Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips
Y Sakai, Y Yoshiura, K Nakazawa - Journal of bioscience and …, 2011 - Elsevier
The proliferation and differentiation properties of embryoid bodies (EB) from mouse
embryonic stem (ES) cells were compared under two microchip conditions: microwell chip
and micropatterned chip. The microwell chip contained 270 microwells (diameter, 600μm;
depth, 600μm) on a polymethylmethacrylate plate and was surface-modified with
polyethylene glycol (PEG) to render it non-adhesive. The micropatterned chip contained 270
gelatin spots (diameter, 200μm) as the cell adhesion area on a glass plate; the region …
embryonic stem (ES) cells were compared under two microchip conditions: microwell chip
and micropatterned chip. The microwell chip contained 270 microwells (diameter, 600μm;
depth, 600μm) on a polymethylmethacrylate plate and was surface-modified with
polyethylene glycol (PEG) to render it non-adhesive. The micropatterned chip contained 270
gelatin spots (diameter, 200μm) as the cell adhesion area on a glass plate; the region …
