[HTML][HTML] Selective chemokine receptor usage by central nervous system myeloid cells in CCR2-red fluorescent protein knock-in mice
N Saederup, AE Cardona, K Croft, M Mizutani… - PloS one, 2010 - journals.plos.org
N Saederup, AE Cardona, K Croft, M Mizutani, AC Cotleur, CL Tsou, RM Ransohoff…
PloS one, 2010•journals.plos.orgBackground Monocyte subpopulations distinguished by differential expression of chemokine
receptors CCR2 and CX3CR1 are difficult to track in vivo, partly due to lack of CCR2
reagents. Methodology/Principal Findings We created CCR2-red fluorescent protein (RFP)
knock-in mice and crossed them with CX3CR1-GFP mice to investigate monocyte subset
trafficking. In mice with experimental autoimmune encephalomyelitis, CCR2 was critical for
efficient intrathecal accumulation and localization of Ly6Chi/CCR2hi monocytes …
receptors CCR2 and CX3CR1 are difficult to track in vivo, partly due to lack of CCR2
reagents. Methodology/Principal Findings We created CCR2-red fluorescent protein (RFP)
knock-in mice and crossed them with CX3CR1-GFP mice to investigate monocyte subset
trafficking. In mice with experimental autoimmune encephalomyelitis, CCR2 was critical for
efficient intrathecal accumulation and localization of Ly6Chi/CCR2hi monocytes …
Background
Monocyte subpopulations distinguished by differential expression of chemokine receptors CCR2 and CX3CR1 are difficult to track in vivo, partly due to lack of CCR2 reagents.
Methodology/Principal Findings
We created CCR2-red fluorescent protein (RFP) knock-in mice and crossed them with CX3CR1-GFP mice to investigate monocyte subset trafficking. In mice with experimental autoimmune encephalomyelitis, CCR2 was critical for efficient intrathecal accumulation and localization of Ly6Chi/CCR2hi monocytes. Surprisingly, neutrophils, not Ly6Clo monocytes, largely replaced Ly6Chi cells in the central nervous system of these mice. CCR2-RFP expression allowed the first unequivocal distinction between infiltrating monocytes/macrophages from resident microglia.
Conclusion/Significance
These results refine the concept of monocyte subsets, provide mechanistic insight about monocyte entry into the central nervous system, and present a novel model for imaging and quantifying inflammatory myeloid populations.
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