Plasma and cerebral spinal fluid tranexamic acid quantitation in cardiopulmonary bypass patients
C Abou-Diwan, RM Sniecinski, F Szlam… - … of Chromatography B, 2011 - Elsevier
A method for the determination of tranexamic acid (TXA) in human plasma and cerebral
spinal fluid (CSF) was developed. Analyses were performed by ultra performance liquid
chromatography with tandem mass spectrometry detection (UPLC–MS/MS) using ɛ-
aminocaproic acid (ACA) as an internal standard. TXA and ACA were extracted from a 50μL
sample of plasma or CSF using a methanol protein crash protocol, and chromatographic
separation was performed on an ACQUITY™ TQD mass spectrometer using a UPLC C18 …
spinal fluid (CSF) was developed. Analyses were performed by ultra performance liquid
chromatography with tandem mass spectrometry detection (UPLC–MS/MS) using ɛ-
aminocaproic acid (ACA) as an internal standard. TXA and ACA were extracted from a 50μL
sample of plasma or CSF using a methanol protein crash protocol, and chromatographic
separation was performed on an ACQUITY™ TQD mass spectrometer using a UPLC C18 …
