Genetic instability of cell lines derived from a single human small cell carcinoma of the lung
SA Engelholm, LL Vindeløv, M Spang-Thomsen… - European Journal of …, 1985 - Elsevier
Specimens from a human small cell carcinoma of the lung were established as a cell line in
vitro. Flow cytometric DNA analysis demonstrated only one tumor cell population in the
parent tumor as well as in the early passages in vitro. After six passages in vitro, two new
subpopulations with different DNA content appeared. By cloning, permanent cell lines were
established from the new subpopulations, whereas the original population stopped growing.
The cloned cell lines were characterized by morphology, chromosomes analysis, electron …
vitro. Flow cytometric DNA analysis demonstrated only one tumor cell population in the
parent tumor as well as in the early passages in vitro. After six passages in vitro, two new
subpopulations with different DNA content appeared. By cloning, permanent cell lines were
established from the new subpopulations, whereas the original population stopped growing.
The cloned cell lines were characterized by morphology, chromosomes analysis, electron …
