[HTML][HTML] The catalytic histidine dyad of high density lipoprotein-associated serum paraoxonase-1 (PON1) is essential for PON1-mediated inhibition of low density …
M Rosenblat, L Gaidukov, O Khersonsky, J Vaya… - Journal of Biological …, 2006 - ASBMB
High density lipoprotein (HDL)-associated paraoxonase-1 (PON1) anti-atherogenic
properties in macrophages, ie inhibition of cell-mediated oxidation of low density lipoprotein
(LDL) and stimulation of cholesterol efflux, were studied using recombinant variants of PON1
and apoA-I expressed in Escherichia coli and reconstituted HDL (rHDL) particles composed
of phosphatidylcholine/free cholesterol (PC/FC) and apoA-I. PON1 lactonase activity is
stimulated by apoA-I by∼ 7-fold relative to PC/FC particles. Wild-type (WT) PON1 bound to …
properties in macrophages, ie inhibition of cell-mediated oxidation of low density lipoprotein
(LDL) and stimulation of cholesterol efflux, were studied using recombinant variants of PON1
and apoA-I expressed in Escherichia coli and reconstituted HDL (rHDL) particles composed
of phosphatidylcholine/free cholesterol (PC/FC) and apoA-I. PON1 lactonase activity is
stimulated by apoA-I by∼ 7-fold relative to PC/FC particles. Wild-type (WT) PON1 bound to …
