Genomic instability in Down syndrome and Fanconi anemia assessed by micronucleus analysis and single-cell gel electrophoresis
SW Maluf, B Erdtmann - Cancer genetics and cytogenetics, 2001 - Elsevier
Cytokinesis-block micronucleus (CB-MN) assay and single-cell gel electrophoresis (SCGE)
were employed to analyze leukocytes from 14 Fanconi anemia (FA) patients, 30 Down
syndrome (DS) patients, and 30 control individuals, to examine the sensitivity of these
techniques to detect genomic instability in these 2 diseases. The DS patients presented
increased DNA damage as measured by SCGE in relation to controls. The frequencies of
micronuclei and dicentric bridges were similar to those of controls. Micronucleus frequency …
were employed to analyze leukocytes from 14 Fanconi anemia (FA) patients, 30 Down
syndrome (DS) patients, and 30 control individuals, to examine the sensitivity of these
techniques to detect genomic instability in these 2 diseases. The DS patients presented
increased DNA damage as measured by SCGE in relation to controls. The frequencies of
micronuclei and dicentric bridges were similar to those of controls. Micronucleus frequency …
