[HTML][HTML] Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin
The glycoprotein IgM is the major antibody produced in the primary immune response to
antigens, circulating in the serum both as a pentamer and a hexamer. Pentameric IgM has a
single J chain, which is absent in the hexamer. The μ (heavy) chain of IgM has five N-linked
glycosylation sites. Asn-171, Asn-332, and Asn-395 are occupied by complex glycans,
whereas Asn-402 and Asn-563 are occupied by oligomannose glycans. The glycosylation of
human polyclonal IgM from serum has been analyzed. IgM was found to contain 23.4 …
antigens, circulating in the serum both as a pentamer and a hexamer. Pentameric IgM has a
single J chain, which is absent in the hexamer. The μ (heavy) chain of IgM has five N-linked
glycosylation sites. Asn-171, Asn-332, and Asn-395 are occupied by complex glycans,
whereas Asn-402 and Asn-563 are occupied by oligomannose glycans. The glycosylation of
human polyclonal IgM from serum has been analyzed. IgM was found to contain 23.4 …