Fluorescence in situ Hybridization (FISH), Basic Principles and Methodology

E Garimberti, S Tosi - Fluorescence in situ Hybridization (FISH) Protocols …, 2010 - Springer
E Garimberti, S Tosi
Fluorescence in situ Hybridization (FISH) Protocols and Applications, 2010Springer
Fluorescence in situ hybridization (FISH) is widely used for the localization of genes and
specific genomic regions on target chromosomes, both in metaphase and interphase cells.
The applications of FISH are not limited to gene mapping or the study of genetic
rearrangements in human diseases. Indeed, FISH is increasingly used to explore the
genome organization in various organisms and extends to the study of animal and plant
biology. We have described the principles and basic methodology of FISH to be applied to …
Abstract
Fluorescence in situ hybridization (FISH) is widely used for the localization of genes and specific genomic regions on target chromosomes, both in metaphase and interphase cells. The applications of FISH are not limited to gene mapping or the study of genetic rearrangements in human diseases. Indeed, FISH is increasingly used to explore the genome organization in various organisms and extends to the study of animal and plant biology. We have described the principles and basic methodology of FISH to be applied to the study of metaphase and interphase chromosomes.
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