MAPK pathway contributes to density-and hypoxia-induced expression of the tumor-associated carbonic anhydrase IX
J Kopacek, M Barathova, F Dequiedt… - … et Biophysica Acta (BBA …, 2005 - Elsevier
Transcription of the CA9 gene coding for a tumor-associated carbonic anhydrase IX (CA IX)
isoform is regulated by hypoxia via the hypoxia-inducible factor 1 (HIF-1) and by high cell
density via the phosphatidylinositol-3-kinase (PI3K) pathway. We examined the role of the
mitogen-activated protein kinase (MAPK) pathway in the control of CA9 gene expression.
Inhibition of MAPK signaling by U0126 in HeLa cells led to reduced activity of the PR1-HRE-
luc CA9 promoter construct and decreased CA IX protein levels in dense culture as well as …
isoform is regulated by hypoxia via the hypoxia-inducible factor 1 (HIF-1) and by high cell
density via the phosphatidylinositol-3-kinase (PI3K) pathway. We examined the role of the
mitogen-activated protein kinase (MAPK) pathway in the control of CA9 gene expression.
Inhibition of MAPK signaling by U0126 in HeLa cells led to reduced activity of the PR1-HRE-
luc CA9 promoter construct and decreased CA IX protein levels in dense culture as well as …