[HTML][HTML] High-throughput functional genomics identifies regulators of primary human beta cell proliferation
The expansion of cells for regenerative therapy will require the genetic dissection of
complex regulatory mechanisms governing the proliferation of non-transformed human cells.
Here, we report the development of a high-throughput RNAi screening strategy specifically
for use in primary cells and demonstrate that silencing the cell cycle-dependent kinase
inhibitors CDKN2C/p18 or CDKN1A/p21 facilitates cell cycle entry of quiescent adult human
pancreatic beta cells. This work identifies p18 and p21 as novel targets for promoting …
complex regulatory mechanisms governing the proliferation of non-transformed human cells.
Here, we report the development of a high-throughput RNAi screening strategy specifically
for use in primary cells and demonstrate that silencing the cell cycle-dependent kinase
inhibitors CDKN2C/p18 or CDKN1A/p21 facilitates cell cycle entry of quiescent adult human
pancreatic beta cells. This work identifies p18 and p21 as novel targets for promoting …