A critical survey of the structure-function of the antisense oligo/RNA heteroduplex as substrate for RNase H
E Zamaratski, PI Pradeepkumar… - Journal of biochemical …, 2001 - Elsevier
The aim of this review is to draw a correlation between the structure of the DNA/RNA hybrid
and its properties as a substrate for the RNase H, as well as to point the crucial structural
requirements for the modified AONs to preserve their RNase H potency. The review is
divided into the following parts:(1) mechanistic considerations,(2) target RNA folding–AON
folding–RNase H assistance in AON/RNA hybrid formation,(3) carbohydrate
modifications,(4) backbone modifications,(5) base modifications,(6) conjugated AONs,(7) …
and its properties as a substrate for the RNase H, as well as to point the crucial structural
requirements for the modified AONs to preserve their RNase H potency. The review is
divided into the following parts:(1) mechanistic considerations,(2) target RNA folding–AON
folding–RNase H assistance in AON/RNA hybrid formation,(3) carbohydrate
modifications,(4) backbone modifications,(5) base modifications,(6) conjugated AONs,(7) …