Broadly neutralizing antibody VRC01 prevents HIV-1 transmission from plasmacytoid dendritic cells to CD4 T lymphocytes
B Su, A Lederle, G Laumond, C Ducloy… - Journal of …, 2014 - Am Soc Microbiol
B Su, A Lederle, G Laumond, C Ducloy, S Schmidt, T Decoville, C Moog
Journal of virology, 2014•Am Soc MicrobiolPlasmacytoid dendritic cells (pDC) poorly replicate human immunodeficiency virus type 1
(HIV-1) but efficiently transfer HIV-1 to adjacent CD4 T lymphocytes. We found that coculture
with T lymphocytes downregulates SAMHD1 expression, enhances HIV-1 replication, and
increases pDC maturation and alpha interferon (IFN-α) secretion. HIV-1 transfer to T
lymphocytes is inhibited by broadly neutralizing antibody VRC01 with efficiency similar to
that of cell-free infection of T lymphocytes. Interestingly, prevention of HIV-1 transmission by …
(HIV-1) but efficiently transfer HIV-1 to adjacent CD4 T lymphocytes. We found that coculture
with T lymphocytes downregulates SAMHD1 expression, enhances HIV-1 replication, and
increases pDC maturation and alpha interferon (IFN-α) secretion. HIV-1 transfer to T
lymphocytes is inhibited by broadly neutralizing antibody VRC01 with efficiency similar to
that of cell-free infection of T lymphocytes. Interestingly, prevention of HIV-1 transmission by …
Abstract
Plasmacytoid dendritic cells (pDC) poorly replicate human immunodeficiency virus type 1 (HIV-1) but efficiently transfer HIV-1 to adjacent CD4 T lymphocytes. We found that coculture with T lymphocytes downregulates SAMHD1 expression, enhances HIV-1 replication, and increases pDC maturation and alpha interferon (IFN-α) secretion. HIV-1 transfer to T lymphocytes is inhibited by broadly neutralizing antibody VRC01 with efficiency similar to that of cell-free infection of T lymphocytes. Interestingly, prevention of HIV-1 transmission by VRC01 retains IFN-α secretion. These results emphasize the multiple functions of VRC01 in protection against HIV-1 acquisition.
American Society for Microbiology