Protection against cartilage proteoglycan synthesis inhibition by antiinterleukin 1 antibodies in experimental arthritis.

FA Van de Loo, OJ Arntz, IG Otterness… - The Journal of …, 1992 - europepmc.org
FA Van de Loo, OJ Arntz, IG Otterness, WB Van den Berg
The Journal of Rheumatology, 1992europepmc.org
We have used neutralizing antibodies raised against murine recombinant interleukin 1 (IL-1)
to demonstrate a role for IL-1 in the cartilage destruction and inflammation of antigen
induced arthritis. Ex vivo production of IL-1 was demonstrated in tissue cultures of joint cross
sections shortly after arthritis induction. Neutralizing antimurine IL-1 antibodies identified the
activity to be about 80% IL-1 alpha 24 h after onset of arthritis. In animals receiving a single
injection of anti-IL-1 antisera at Day-3, cartilage proteoglycan synthesis suppression during …
We have used neutralizing antibodies raised against murine recombinant interleukin 1 (IL-1) to demonstrate a role for IL-1 in the cartilage destruction and inflammation of antigen induced arthritis. Ex vivo production of IL-1 was demonstrated in tissue cultures of joint cross sections shortly after arthritis induction. Neutralizing antimurine IL-1 antibodies identified the activity to be about 80% IL-1 alpha 24 h after onset of arthritis. In animals receiving a single injection of anti-IL-1 antisera at Day-3, cartilage proteoglycan synthesis suppression during the first 2 days of arthritis was prevented. Normal proteoglycan synthesis was maintained until Day 4 when anti-IL-1 antisera was given at Days-2, 0, and 2 or arthritis. Dose response experiments showed that the reduction in inflammation was insufficient to account for the clearcut reduction in cartilage proteoglycan synthesis inhibition. Our results demonstrate that IL-1 plays a role in cartilage pathology in murine antigen induced arthritis.
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