Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay
JY Koh, DW Choi - Journal of neuroscience methods, 1987 - Elsevier
JY Koh, DW Choi
Journal of neuroscience methods, 1987•ElsevierMeasurement of lactate dehydrogenase (LDH) activity released to the extracellular bathing
media has been found to be a simple yet quantitative method for assessing glutamate
mediated central neuronal cell injury in cortical cell culture. Extracellular LDH is both
chemically and biologically stable; the magnitude of LDH efflux in the cultures correlates in a
linear fashion with the number of neurons damaged by glutamate exposure.
media has been found to be a simple yet quantitative method for assessing glutamate
mediated central neuronal cell injury in cortical cell culture. Extracellular LDH is both
chemically and biologically stable; the magnitude of LDH efflux in the cultures correlates in a
linear fashion with the number of neurons damaged by glutamate exposure.
Abstract
Measurement of lactate dehydrogenase (LDH) activity released to the extracellular bathing media has been found to be a simple yet quantitative method for assessing glutamate mediated central neuronal cell injury in cortical cell culture. Extracellular LDH is both chemically and biologically stable; the magnitude of LDH efflux in the cultures correlates in a linear fashion with the number of neurons damaged by glutamate exposure.
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