Organoids as model for infectious diseases: culture of human and murine stomach organoids and microinjection of Helicobacter pylori
S Bartfeld, H Clevers - JoVE (Journal of Visualized Experiments), 2015 - jove.com
Recently infection biologists have employed stem cell derived cultures to answer the need
for new and better models to study host-pathogen interactions. Three cellular sources have
been used: Embryonic stem cells (ESC), induced pluripotent stem cells (iPSC) or adult stem
cells. Here, culture of mouse and human gastric organoids derived from adult stem cells is
described and used for infection with the gastric pathogen Helicobacter pylori. Human
gastric glands are isolated from resection material, seeded in a basement matrix and …
for new and better models to study host-pathogen interactions. Three cellular sources have
been used: Embryonic stem cells (ESC), induced pluripotent stem cells (iPSC) or adult stem
cells. Here, culture of mouse and human gastric organoids derived from adult stem cells is
described and used for infection with the gastric pathogen Helicobacter pylori. Human
gastric glands are isolated from resection material, seeded in a basement matrix and …
