[HTML][HTML] The interface between catalytic and hemopexin domains in matrix metalloproteinase-1 conceals a collagen binding exosite
LH Arnold, LE Butt, SH Prior, CM Read… - Journal of Biological …, 2011 - ASBMB
Matrix metalloproteinase-1 (MMP-1) is an instigator of collagenolysis, the catabolism of triple
helical collagen. Previous studies have implicated its hemopexin (HPX) domain in binding
and possibly destabilizing the collagen substrate in preparation for hydrolysis of the
polypeptide backbone by the catalytic (CAT) domain. Here, we use biophysical methods to
study the complex formed between the MMP-1 HPX domain and a synthetic triple helical
peptide (THP) that encompasses the MMP-1 cleavage site of the collagen α1 (I) chain. The …
helical collagen. Previous studies have implicated its hemopexin (HPX) domain in binding
and possibly destabilizing the collagen substrate in preparation for hydrolysis of the
polypeptide backbone by the catalytic (CAT) domain. Here, we use biophysical methods to
study the complex formed between the MMP-1 HPX domain and a synthetic triple helical
peptide (THP) that encompasses the MMP-1 cleavage site of the collagen α1 (I) chain. The …
