[PDF][PDF] Activation domain–mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays
KE Neely, AH Hassan, AE Wallberg, DJ Steger… - Molecular cell, 1999 - cell.com
Molecular cell, 1999•cell.com
The yeast SWI/SNF complex is required for the transcription of several yeast genes and has
been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we
show that the complex stimulated in vitro transcription from nucleosome templates in an
activation domain–dependent manner. Transcription stimulation by SWI/SNF required an
activation domain with which it directly interacts. The acidic activation domains of VP16,
Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the …
been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we
show that the complex stimulated in vitro transcription from nucleosome templates in an
activation domain–dependent manner. Transcription stimulation by SWI/SNF required an
activation domain with which it directly interacts. The acidic activation domains of VP16,
Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the …
Abstract
The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain–dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.
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